Saturday, September 8, 2018

DNA FINGER PRINTING


DNA FINGER PRINTSING : The technique of DNA fingerprinting was developed for the first time (1985,86) by Alec Jeffrey and his colleagues at Leicester university in U.K. in this field, establishment of the identity of a person with the help of blood stains, semen (sperm) stains or hair roots will be possible with almost absolute certainty. In this technique, DNA will be isolated from blood stains, semen stains or hair roots and will be subjected to southern blotting. DNA hybridization with the help of specific DNA probes. This will reveal polymorphism In DNA, which has a very stable inheritance. For this purpose, DNA from blood,semen or urine stains may also be amplified using pcr technique.

 It is speculated that the above technique will allow the identification of rapisists in rape cases, and of mother and or father in case of doubtful percentage. This technique will allow identification even which the stains on victims clothes etc.  Are several years old and with much more certainty than has hitherto been possible through technique of blood group etc. Since the number of blood groups available becomes a limitation. The technique of DNA fingerprinting reveals such a great polymorphism that the possibility of two persons having same pattern of DNA fingerprinting is very remote. 

In India, DNA fingerprinting tests are carried out at the center for cell and molecular biology(CCMB), Hyderabad. For this purpose, a test with the bkm probe (banded krait minor satellite DNA) earlier used for identification of sex chromosomes has been found to cost one-tenth of the cost of tests used in Europe and U.S.A. paternity dispute cases are much more common in India and most of them are referred to ccmb for DNA evidence. The first test on DNA fingerprinting was used in June, 1989 to settle a drawn-out paternity case in madras. 

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